蜂膠在培養CD4 +淋巴細胞中展現抗HIV-1活性

治療人類免疫缺陷病毒1型（HIV-1）感染迫切需要額外的藥物，導致我們評估天然產物蜂膠在CD4 +淋巴細胞和小膠質細胞培養物中的抗HIV-1活性。製備蜂膠20％乙醇提取物, 透過蜂膠濃度控制之方式來表達抑制病毒的成效（分別在CD4 +和小膠質細胞培養物中蜂膠用量在66.6μg / ml觀察到最大抑制效果為85％和98％）。從幾個地理區域的蜂膠樣品觀察到類似的抗HIV-1活性。CD4 +淋巴細胞中蜂膠抗病毒性質的機制似乎部分涉及病毒進入的抑制。雖然蜂膠對逆轉錄酶抑製劑zidovudine具有附加的抗病毒作用，對蛋白酶抑制劑沒有顯著影響。該體外研究的結果支持蜂膠或其一種或多種組成分，用在治療HIV-1感染中的臨床試驗的需要。
https://doi.org/10.1016/j.jep.2005.05.045Get rights and content
關鍵詞
蜂膠、蜜蜂、HIV-1、艾滋病


An urgent need for additional agents to treat human immunodeficiency virus type 1 (HIV-1) infection led us to assess the anti-HIV-1 activity of the natural product propolis in CD4+ lymphocytes and microglial cell cultures. Propolis inhibited viral expression in a concentration-dependent manner (maximal suppression of 85 and 98% was observed at 66.6 μg/ml propolis in CD4+ and microglial cell cultures, respectively). Similar anti-HIV-1 activity was observed with propolis samples from several geographic regions. The mechanism of propolis antiviral property in CD4+ lymphocytes appeared to involve, in part, inhibition of viral entry. While propolis had an additive antiviral effect on the reverse transcriptase inhibitor zidovudine, it had no noticeable effect on the protease inhibitor indinavir. The results of this in vitro study support the need for clinical trials of propolis or one or more of its components in the treatment of HIV-1 infection.

Keywords
Propolis, Honey bees, HIV-1, AIDS